Journal: Virus Research

Article Title: Propagation and immunological characterization of coxsackievirus A10 in a serum-free HEK293A cell culture system

doi: 10.1016/j.virusres.2023.199101

Figure Lengend Snippet: Propagation profiles of CVA10 in serum-free roller bottle culture. HEK293A cells were infected with CVA10 at an MOI of 10 −4 . (A) Morphology of serum-free HEK293A cells in roller bottle. (B) Morphology of HEK293A cells after CVA10 infection. (C) TCID 50 values of three roller bottles after counting CPE in infected RD cells. The TCID 50 values were calculated using the Reed-Muench method. The bar represents 100 µm.

Article Snippet: Rhabdomyosarcoma (RD) cells and Madin-Darby Canine Kidney (MDCK) cells were obtained from the Bioresource Collection and Research Center (BCRC), Hsinchu, Taiwan.

Techniques: Infection, Endpoint Dilution Assay

Journal: Virus Research

Article Title: Propagation and immunological characterization of coxsackievirus A10 in a serum-free HEK293A cell culture system

doi: 10.1016/j.virusres.2023.199101

Figure Lengend Snippet: Propagation profiles of CVA10 in serum-free suspension culture. HEK293A cells were infected with CVA10 at an MOI of 10 −4 . (A) Suspension HEK293A cells in a spinner. (B) Suspension HEK293A cells after CVA10 infection. (C) TCID 50 values of four spinners after counting CPE in the infected RD cells. (D) Extracellular and intracellular TCID 50 values of serum-free bioreactor culture after counting CPE in infected RD cells. The TCID 50 values were calculated using the Reed-Muench method. The bar represents 100 µm.

Article Snippet: Rhabdomyosarcoma (RD) cells and Madin-Darby Canine Kidney (MDCK) cells were obtained from the Bioresource Collection and Research Center (BCRC), Hsinchu, Taiwan.

Techniques: Suspension, Infection, Endpoint Dilution Assay

Journal: Virus Research

Article Title: Propagation and immunological characterization of coxsackievirus A10 in a serum-free HEK293A cell culture system

doi: 10.1016/j.virusres.2023.199101

Figure Lengend Snippet: Propagation profiles of other HFMD-related viruses in serum-free HEK293A cell culture. HEK293A cells were infected with each virus (CVA6 and EV-A71) at MOI = 10 −4 . The culture and harvest conditions were identical to that described in the Method for CVA10 propagation. (A) Morphology of HEK293A cells after virus infection in roller bottle at 6 DPI. (B) Morphology of suspension HEK293A cells after virus infection in spinner at 4 DPI. (C) TCID 50 values of serum-free roller bottle cultures after counting CPE in infected RD cells. (D) TCID 50 values of serum-free spinner cultures after counting CPE in infected RD cells. The TCID 50 values were calculated using the Reed-Muench method. The bar represents 100 µm.

Article Snippet: Rhabdomyosarcoma (RD) cells and Madin-Darby Canine Kidney (MDCK) cells were obtained from the Bioresource Collection and Research Center (BCRC), Hsinchu, Taiwan.

Techniques: Cell Culture, Infection, Virus, Suspension, Endpoint Dilution Assay

Journal: BMC Cancer

Article Title: An aza-macrocycle containing maltolic side-arms (maltonis) as potential drug against human pediatric sarcomas

doi: 10.1186/1471-2407-14-137

Figure Lengend Snippet: Malten and maltonis effect on DNA structure and sarcoma cell growth. (A) Effects of the two compounds on electrophoretic migration of plasmid DNA. After 2 hours incubation in presence of 4 mM malten, 4 mM maltonis or 25 μM CDDP, circular plasmid DNA (pLL3.7) was separated by agarose gel electrophoresis. Supercoiled (white arrow), open circular (black arrow) plasmid form and high molecular weight DNA complexes formation (black bar) are indicated. (B) PCR inhibition assay. Amplification delay (folds) was calculated for each set of primers (for details see Additional file ) as the difference between the Ct values of treated and untreated samples. Graphical representation of the exponential decrease of the number of amplifiable DNA sequences after incubation with malten, maltonis or CDDP. (C) Histogram showing IC50 values for malten (light bars) and maltonis (dark bars) in a panel of sarcoma cell lines. RMS, rhabdomyosarcoma; OS, osteosarcoma; ES, Ewing sarcoma. Values are expressed as mean of three independent experiments ± SE. (D) Maltol derived compounds inhibit tumour growth in anchorage-independent conditions: columns are the mean of three independent experiments ± SE performed on RD/18 (seeded cells: 10,000/dish), U-2OS(seeded cells: 10,000/dish) and TC-71 (seeded cells: 3,300/dish) cells. Statistical analysis was performed by Student’s t test: *P < 0.05; **P < 0.01. (E) Fold increase in drug resistance to conventional drugs (light grey) or to maltonis (black): fold resistance is calculated on the values of IC50 of each resistant cell line against its respective sensitive parental one. Values are representative of three independent experiments.

Article Snippet: The RD/18 cell line is a clone of the commercially available human embryonal rhabdomyosarcoma cell line RD (Flow Laboratories), obtained at the Cancer Research Section, University of Bologna, Bologna, Italy [ ].

Techniques: Migration, Plasmid Preparation, Incubation, Agarose Gel Electrophoresis, High Molecular Weight, Inhibition, Amplification, Derivative Assay

Journal: BMC Cancer

Article Title: An aza-macrocycle containing maltolic side-arms (maltonis) as potential drug against human pediatric sarcomas

doi: 10.1186/1471-2407-14-137

Figure Lengend Snippet: Maltonis mean IC50 values in a panel of human derived sarcoma and human normal mesenchymal cells

Article Snippet: The RD/18 cell line is a clone of the commercially available human embryonal rhabdomyosarcoma cell line RD (Flow Laboratories), obtained at the Cancer Research Section, University of Bologna, Bologna, Italy [ ].

Techniques: Derivative Assay